Chlorine dioxide and chlorine effectiveness to prevent Escherichia coli O157:H7 and Salmonella cross-contamination on fresh-cut Red Chard

http://ucanr.edu/datastoreFiles/234-2461.pdf

Chlorine dioxide and chlorine effectiveness to prevent Escherichia coli O157:H7 and Salmonella cross-contamination on fresh-cut Red Chard 


Alejandro Tomás-Callejas a , Francisco López-Gálvez b , Adrian Sbodio c , Francisco Artés a , Francisco Artés-Hernández a , Trevor V. Suslow c,* a Postharvest and Refrigeration Group, Department of Food Engineering, Technical University of Cartagena, Paseo Alfonso XIII 48, Cartagena, Murcia 30203, Spain b Research Group on Quality, Safety and Bioactivity of Plant Foods, CEBAS-CSIC, P.O. Box 164, Espinardo, Murcia 30100, Spain cDepartment of Plant Sciences, University of California, One Shields Avenue, Davis, CA 95616, USA

abstract
Washing procedures applied to fresh produce have the potential to reduce contamination from the surface of the product. However, the wash water may also serve as a source of contamination or has great potential to result in cross-contamination. The objective of this study was to evaluate process handling cross-contamination potential and pathogen removal of initially low numbers of attached cells of Salmonella and Escherichia coli O157:H7 during the washing-disinfection, rinsing, and de-watering steps of fresh-cut Red Chard baby leaves as affected by NaClO and ClO2. The efficacy and stability of liquid ClO2 applied to the water as the disinfectant treatment was also evaluated. Non-inoculated leaves were mixed with inoculated leaves (about 3e5% of total weight) and processed as a unit. After processing, no confirmed colonies on selective media were recovered from the non-inoculated leaves and qPCR was used for detection below the limit of quantitative recovery. ClO2 substantially prevented E. coli O157:H7 cross-contamination but was not effective for the inoculated Salmonella. Large populations of Salmonella were recovered from centrifugation discharge effluent water whereas no colonies were detected from water in contact with inoculated leaves collected from preceding washing unit operations. At an industrial level, this represents a potential risk of cross-contamination to product and equipment at the step immediately prior to packaging. These results suggest that the centrifugation effluent water could be used as a potential sample point to evaluate lot contamination and cross-contamination in the processing chain, even at low levels of pathogens, as were used in this study, undetectable by conventional sampling methods